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KMID : 0545120080180101735
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 10 p.1735 ~ p.1740
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Isolation and Characterization of PERV-C env from Domestic Pig in Korea
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Park Sung-Han
Bae Eun-Hye
Park Sang-Min
Park Jin-Woo
Lim Mi-Suk
Jung Yong-Tae
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Abstract
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Clone PERV-C (A3) env was isolated from the genomic DNA of domestic pig (Sus scrofa domesticus) in Korea to investigate the molecular properties of PERV-C. The nucleic acid homologies between the PERV-MSL (type C) reference and the PERV-C(A3) clone was 99% for env, but a single base pair deletion was found in the transmembrane (TM) region of the env open reading frame. To examine the functional characteristics of truncated PERV-C env, we constructed a replication-incompetent retroviral vector by replacing the env gene of the pCL-Eco retrovirus vector with PERV-C env. A retroviral vector bearing PERV-C/A chimeric envelopes was also created to complement the TM defect. Our results indicated that truncated PERV-C env was not infectious in human cells as expected. Interestingly, however, the vector with the PERV-C/A envelope was able to infect 293 cells. This observation suggests that recombination within PERV-C TM could render PERV-C infectious in humans. To further characterize PERV-C/A envelopes, we constructed an infectious molecular clone by using a PCR-based technique. This infectious molecular clone will be useful to examine more specific regions that are critical for human cell tropism.
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KEYWORD
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PERV-C, pseudotype assay, xenotransplantation, envelope
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